Metabonomics Study on Intervention Effects of Ginsenosides on Energy Metabolism of Day-night Reversal Stress Rats
人参皂苷对昼夜颠倒应激大鼠能量代谢干预作用的代谢组学研究

This article was aimed to study the influence of day-night reversal on rat energy metabolism, and evaluate the intervention effect of ginsenosides on day-night reversal stressed rats. A total of 24 rats were divided into the normal control group, day-night reversal model group, and ginsenosides (100 mg/kg) intervention group. After 1 week accommodate, model group and ginsenosides intervention group were made day-night reversal for 8 days. The body weight of rats in each group was weighed every day before/after experiment. And the food intake of rats in each group was weighed on the day before the experiment and on the 8th day of the experiment. At the end of 8th day, serum samples were derivatized with TMCS prior to GC-MS instrumental analysis. And partial least squares-discriminant analysis (PLS-DA) was used to find the potential biomarkers, and evaluate the intervention effects of ginsenosides. The results showed that the day-night reversal stress improved body weight and food intake of the rat markedly (P < 0.05). Compared to normal control group, the amount of food intake appeared reversal in day and night. Ginsenosides lightly inhibit the increase of body weight and food intake. Metabolomics studies showed that day-night reversal stress had connection with changes in energy metabolism, protein absorption and degradation, fatty acid metabolism, and cholesterol metabolism pathway. And ginsenosides had some improvement. It was concluded that ginsenosides can modulate excitatory and inhibitive neurotransmitters in upstream metabolism pathway, and change the substances in downstream, then improve day-night reversal induced metabolism changes.