A preliminary study on conservation of Andrias davidanus semen in vitro
大鲵精液离体保存的初步研究

Gonadal development of Andrias davidanus not synchronized,semen characteristics and preservation conditions of A.davidanus were studied targeting the increase of fertility rate.Semina were collected by artificial assistance approach,and a regular assay was conducted on the basic features such as semen volume,pH,density,vitality,etc.Preservation conditions,namely,protection medium,illumination and temperature,were also studied.The results showed that semen volume of pro-male A.davidanus was up to 12 mL/kg,pH 6.7-7.0 of subacid condition;and that the semen density was(2.15-10.75)million/mL,sperm motility 0.7-0.9.In vitro conservation conditions,dark environment at ambient illuminance(20 lx).The result also indicated that the survival time of the semina was 0.5 h longer than those conserved in the luminous environment(400 lx);low temperature(0-4℃) could extend the survival time of the semina 3d longer than those at room temperature(21-22℃) in dark environment;in the cold dark environment,semina with protection medium added in could extend the survival time 6-12 h longer than the control group.