Effect of Some Nutrients on the Growth and Haemolytic Toxins Production of Chattonella marina
营养盐对海洋卡盾藻生长与产毒的影响

Chattonella marina is one of the most toxic red tide organisms. The hemolytic toxins and reactive oxygen species generated from C. marina could be the main cause for the death of the fish. To establish the mechanism on the formation of the haemolytic toxins, the effects of nitrogen, phosphate and iron salts on the growth and toxin of C. marina have been investigated. The results show that iron and nitrogen salts have significant effects on the growth of C. marina, while nitrogen and phosphate salts have significant effects on the haemolytic toxin production of C. marina. Of all the culture conditions, in iron concentration of 0, 0.492, 4.92 and 24.6mg·L-1, nitrogen concentration of 0, 7.5, 75 and 375mg·L-1, and phosphonium concentration of 0, 0.5, 5 and 25mg·L-1, the growth rate was maximal when the concentration of three nutrients were as followed: iron concentration of 0.492mg·L-1, nitrogen concentration of 375mg·L-1, phosphonium concentration of 5mg·L-1; while the maximal toxin production was in iron concentration of 0mg·L-1, nitrogen concentration of 375mg·L-1, phosphonium concentration of 0.5mg·L-1. The results suggest that the high nitrogen and low phosphate salts can accelerate C. marina to synthesize the hemolytic toxins.