URACE-ENHANCED RAMAN SPECTROSCOPY OF BOMBYX MORI VIRUS ADSORBED ON COLLOIDAL SILVER
家蚕病毒的表面增强拉曼光谱研究

The Surface-enhanced Raman spectla of the incluSion body of Bombyx sari NuclearPolyhdssis Virus (BmNPB) and Bombyx mori Cytoplasmic Polyhdssis Virus (BmCPB) absorbed on colloidal silVer were obtained and analed. The BInNI,B and BmCPB were foundto adsorb on the silver sauce through the sulphur atom and also the carboxylate and ammino groups. The BmCPB adsobo on colloidal silver has tans-gauche-tians confonnation ofC-C-S-S-C-C linkage as wb as bans and gauche confonnation around the S-CH2,and CH2-CH2 bonds. But the BmNPB adsortal on colloidal silver has gauche and gaucheconfonnahon around the S-CH2 and CH2-CH2 bonds. All tryptophan asidues in theBmNPB and BmCPB are buried in the hydrophobic environment.