Recombinant human gapM1 expressed in Pichia pastoris and its anti-diabetic effect
重组人球状脂联素在毕赤酵母中的表达和生物学活性 分析

Adiponectin is an adipokine predominantly synthesized and secreted by adipocytes in the white adipose tissue,and it can lower the blood glucose level and increase free fatty acid oxidation.In the current study,we developed the globular domain of adiponectin(gapM1) to treat type II diabetes.In both flask and fermentor,we cultivated Pichia pastoris expressing recombinant gapM1 and established the purification procedure by using gel filtration and anion exchange chromatography.To evaluate the biological activi...