Research of Real-time Fluorescent PCR in the Rapid Differential Detection of H5、 H9、 H7 Subtype Avian Influenza Inactivated Vaccines
实时荧光PCR技术快速鉴别检测H5、H9、H7亚型禽流感灭活疫苗的研究

Specific primers and TaqMan MGB probes were designed with Primer Express 2.0 software according to the conserved region of the H5,H9,H7 subtype AIV hemagglutinin gene to make research of real-time fluorescent one-step PCR in the differential detection of H5,H9,H7 subtype avian influenza inactivated vaccines.The result showed that the method was specific and reproducible.No cross-reaction was discovered with other avian disease vaccines.Real-time fluorescent PCR provided a specific,sensitive,rapid and convenient method for the subtype identification of avian influenza inactivated vaccines.