Construction of gold film solid-phase carrier of myoglobin monoclonal antibody based on thiol self-assembly
基于硫醇自组装的肌红蛋白单克隆抗体金膜固相载体的构建

In order to immobilize myoglobin(Mb) monoclonal antibody on gold film solid-phase carrier,we grew a mixed self-assembled monolayers(SAMs) of acid thiol and mercapto ethanol on gold film.Then we analyzed the property of the sample by atomic force microscopy and X-ray photoelectron spectroscopy.Then,we used 1-(3-dimethyl aminopropyl)-3-ethyl carbodiimide hydrochloride as catalyst to couple SAMs with amino of antibody so that we immobilized antibody on surface of gold film,followed by detecting myoglobin antig...