Establishment and Optimization of the Regeneration System for Common Dandelion (Taraxacum officinale Weber)
药蒲公英再生体系的建立和优化

A protocol is presented for direct and indirect regeneration of common dandelion (Taraxacum officinale Weber) from leaf and petiole explants. Mult iple shoots were obtained on MS medium containing 0.2 mg/L IAA and 1 mg/L TDZ. For i ndirect regeneration, fragile calli were obtained from leaf and petiole explants on MS medium supplemented with 0.5 mg/L 2,4-D and 2.0 mg/L 6-BA. Regenerate d pl antlets were obtained when these calli were cultured on MS medium containing 1. 0 mg/L 6-BA. Random amplified polymorphic DNA (RAPD) analysis of nine regenerated plantlets revealed 61 scorable bands from 10 primers, including three specific b ands.