Cloning and Expression of MGMT cDNA and Analysis of the DNA Repair Activity of the Recombinant Protein
人MGMT基因Cdna的克隆表达及表达蛋白修复功能的鉴定

A cDNA encoding human O6-methylguanine-DNA methyltranferase (MGMT) was cloned from Hela S3 cells and the sequence is identical with the published data.The MGMT cDNA was inserted into the expression vector pET-21a and transformed into \%E.coli\% BL21 (DE3).A 24 kD protein was expressed after IPTG induction.Essays using lethal dose of alkylating agents indicate that expression of MGMT protein can repair the DNA mutations of the recombinant bacteria produced by alkylating agents.