ANALYSIS ON EXPRESSION AND FUNCTION OF CD39 IN HEMATOPOIETIC CELL LINES
血源细胞系中CD39的表达和功能分析

Extracellular ATP mediates intercellular communication through activation of P2 receptors. CD39 is an Ecto-(Ca2 , Mg2 )-apyrase, which hydrolyzes ATP and ADP. However, how the CD39 modulates ATP-mediated signal? The mechanism remains largely unknown. In this study, semi-quantitative RT-PCR, ABC immunohistochemistry assay, flow cytometry analysis and luciferase/luciferin assay were used to study the expression and function of CD39 in seven hematopoietic cell lines. The results showed that the expression of CD39 varied among these cell lines; CD39 was expressed in both J6-1 and LCL-H at high levels, in HL60 cells at low level, while in Namalva, Jurkat and U937 cells at very low levels. Furthermore, the expression pattern of CD39 was consistent with the results of cell surface ATPase activity and constitutive levels of extracellular ATP of these cells. Our results indicated that CD39 contributed to the ATPase activity on the surface of these hematopoietic cells, and suggested that CD39 might play an important role in the modulation of intercellular communication through P2 receptors.