Construction and Expression of Human Micro Plasminogen Gene in Escherichia coli
人微小纤溶酶原基因的克隆和表达

Human mPlg was amplified by PCR, using pBS S1 cDNA encoding human plasmingen as a templete. The mPlg gene was cloned into expression vector and transfected into E.coli JF1125. SDS PAGE analysis revealed that expression product was Mr.29 and about 24% of total bacteria protein and formed inclusion body. After refoding by Air Oxidation and Cysteine Reoxidation, the fibrinolysis activity of r mPlg was shown. Effects of protein concentration and refolding time on renaturation efficiency were discuss.