Cloning and Expression of 21.7kD Protein Gene of Sch istosoma japonicum(Chinese strain)
日本血吸虫中国大陆株21.7kD蛋白编码基因的克隆和表达

A 558 bp cDNA fragment was amplified by RT-PCR from adult Schistosoma japonicum(Chinese strain) mRNA with a pair of primers that were designed according to published Sj21.7p gene encoding 21.7 kD protein of Schistosoma japonicum(Philippines strain). Sequence analysis indicated that this frame, named Sj21.7(Ch), with 99% homology to Sj21.7p, contained a complete open reading fragment (ORF) of 21.7 kD protein gene of Schistosoma japonicum(Chinese strain). The amino acid sequence shared 98% homology with 21.7 kD protein of Schistosoma japonicum. This fragment was cloned into the expression vector pET28a (+) and subsequently expressed in Escherichia coli with IPTG induction. SDS-PAGE analysis revealed that the molecular weight of this expressed product was 25.4 kD.Western blotting showed that the recombinant protein reacted well with the rabbit serum immunized with Sj worm antigen,indicating that this expressed product had good antigenicity.