INTERACTION OF DNA WITH PEROXIDIZING ERYTHROCYTE MEMBRANE
DNA与红细胞膜脂质过氧化相互作用研究

Interaction of DNA with peroxidizing erythrocyte membrane was studied. Compated with the system without DNA, the addition of DNA obviously enhance the fluorescence intensity at 440nm.It is due to the formation of new fluorescence product between DNA and peroxidizing erythrocyte membrane, with maximum excitation wavelength at 315um and maximum emission wavelength at 410nm. Vitamin E strongly inhibital the formation of fluorescence product. After DNA was separated and purified, and EB was used as fluoascent probe to detect the higher structure of DNA we found that fluorescent spectrum of DNA-EB changed obviously, and the energy transfer effichacy from DNA tO EB decreased , this showed that DNA was damaged. Among the four usual bases, guanine damage was much more serious than those of the other three.