Studies on High Density Cultivation of Vero Cells with Microcarriers
微载体高密度培养Vero细胞的研究

First of all, Biosilon have been chosen from eight kinds of solid microcarriers as the most suitable one for high density cultivation of Vero cells, because it has some advantages than others ,such as it can be reused and its price was the lowest. Then some conditions affecting high density cultivation of Vero cells including microcarrier concentration, inoculum density and gassing were studied with a 500ml modified Wheaton spinner bottle. When the inoculum cell density was 9.24x105/ml, the cell density could reach 2. 34x107/ml after 25 days of cultivation, it showed that Biosilon microcarrier concentration of 50-60mg/ml had no toxicity at all on cells. When cultivation with 1.5L CelliGen bioreactor at the cell inoculum density of 4.98x105/ml,and the perfusion volume was increased from 0.20 to 3. 65L/d gradually, finally the cell density could reach 2. 5x107/rnl after 22 days of cultivation. When vesicular stomatitis virus (VSV) was inoculated into the culture of 1. 21x106/ml at the same MOI (multiplicity of infection), the virus titer reached 7log TCID50/ml and Slog TCID50/ml respectively.