CONSTRUCTION OF SUBSTRACTED cDNA LIBRARY OF LITOPENAEUS VANNAMEI INDUCED BY VIBRIO ALGINOLYTICUS AND ANALYSIS OF EXPRESSED SEQUENCE TAGS
溶藻弧菌诱导凡纳滨对虾消减文库的构建及其表达序列标签分析

Vibrio alginolyticus is one of the main causative agents resulting in serious infectious diseases of Litopenaeus vannamei and other animals.Suppression subtractive hybridization(SSH) is a new and highly effective method for analysing differentially expressed or tissue-specific cDNA probes and libraries.It is applicable to diagnosis of disease,development,tissue specificity,or other differential expressing.Based on the user manual of Clontech PCR-SelectTM cDNA subtraction kit,a suppression subtraction cDNA l...