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水生生物学报 2010
STUDY ON TRYPTASE IN THE MAST CELL IN THE DIGESTIVE TRACT OF INDIAN BULLFROG(RANA TIGRINA RUGULOSA) BY AN IMMUNOHISTOCHEMICAL METHOD
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Abstract:
A murine monoclonal antibody(AA1) raised against human mast cell tryptase was used in this experiment to investigate the possibility existence of tryptase-positive mast cells of the digestive tract tissue which collected from Indian bullfrog(Rana tigrina rugulosa) by ElivisionTMplus immunohistochemical techniques.To prove its reliability,Alcian blue-Safranin O staining and modified toluidine blue staining was compared with this method when gastric cancer tissue from an adult man was used as a positive contr...