Studies of hemocytes DNA damage by two pesticides acetamiprid and chlorpyrifos in predaceous spiders of Pardosa astrigera Koch
杀虫剂啶虫脒和毒死蜱对捕食蜘蛛血细胞DNA的损伤作用

This study was initiated to combine micronucleus test (MN) and comet assay to assess the DNA damage of the two pesticides on the Pardosa astrigera Koch. The objective was to achieve a more comprehensive understanding of the effects and potential risks of the pesticides on the spiders. The results showed that there were differences in the frequencies of micronuclei when each concentrations of acetamiprid and chlorpyrifos (r<0.05 orr<0.01), compared with the control groups. And the dose-effect relationship was observed evidently (r=0.9843).The frequencies of micronuclei in cephalothorax and abdomen hemocytes of Pardosa astrigera were observed for both acetamiprid (r=0.9284, r=0.9071) and chlorpyrifos (r=0.9841, r=0.9793). The results of the comet assay showed that the distributions in the damage grades in all the pesticide-treated groups were significantly different from the control (p< 0.01).DNA damage scores expressed as arbitrary units (AUs) increased with the exposure levels of the two pesticides and in the tests dose-effect relationships were observed, the DNA damage in cephalothorax and abdomen hemocytes of Pardosa astrigera were observed for both acetamiprid (r=0.9838, r=0.9834) and chlorpyrifos (r=0.9807, r=0.9659). The frequencies of micronuclei and DNA damage of the abdomen hemocytes of Pardosa astrigera is more serious than the cephalothorax. MN test and comet assay in this study provided valuable information to evaluate the pesticides risks to natural enemies and