Expresión de las moléculas de adhesión en la anemia drepanocítica

in patients with drepanocytemia (dp) in basal state and with crises of painful vaso-occlusive (pvoc) authors studied the expression in mononuclear cells (mnc) and neutrophils, of lfa-1, vla-4, l-selectine and icam-1; in peripheral blood (pb), vcam-1 and cd34 molecules, and in red blood cells with different density, vla-4 and lutheran molecules, with specific monoclonal antibodies by indirect immunofluorescence by flux cytometry. in both groups there was a significant decrease in expression of lfa-1 and vla-4 molecules mnc and of lfa-1 in neutrophils of patients basal state; decrease in l-selectine molecule expression in mnc and in patients in basal state. increase of iacm-1 (cd54), cd34 and vcam-1 (cd106) in mnc and in peripheral blood of basal patients shows the presence of a permanent leukocyte activation state determined by the continuous subclinical inflammation. the significant increase of icam-1 in neutrophils and cd34 and vcam-1 in peripheral blood in both groups of patients with a higher increase of vcam-1 in patients in crises, shows the monocyte, lymphocyte , neutrophils, and of endothelial cells during the pvoc and the release of these latter to periphery. we note a higher expression of lfa-1, l-selectine and icam-1 molecules in neutrophils of patients with pvoc and a significant increase of cd18 molecule in mnc of patients with pvoc that may be explained by monocyte activation in vaso-occlusive phenomenon.