Improved Overall Bone Health Activity in MG-63 Cell Line after Biofield Energy Healing Treatment on Vitamin D3.

The aim of the study is to evaluate the role of The Trivedi Effect？-Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health parameters in vitro using MG-63 cells. The parameters of bone health tested are alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization. The test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Kathleen Starr Vagt and samples were defined as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay exhibited increased cell viability more than 72% with safe and nontoxic profile among test samples on MG-63 cell line. ALP was significantly increased by 140.8% (50 μg/mL), 167.3% (100 μg/mL),and 223.5% (50 μg/mL) in the UT-DMEM BT-TI, BT-DMEM UT-TI, and BT-DMEM BT-TI groups, respectively as compared with the untreated group. The level of collagen was significantly increased by 120.7% and 93% at 1 and 10 μg/mL, respectively in the UT-DMEM BT-TI group, while 46.7%, 100.9%, and 29.2% at 1, 10, and 50 μg/mL, respectively in BT-DMEM UT-TI group as compared with the untreated group. In addition, BT-DMEM BT-TI group showed a significant increased collagen level by 60.9%, 93%, and 14.4% at 1, 10, and 50 μg/mL, respectively as compared with the untreated test item and DMEM group. The percent of bone mineralization was significantly increased by 95.9% at 100 μg/mL in the UT-DMEM BT-TI group, while 162% and 35.5% at 50 and 100 μg/mL, respectively in the BT-DMEM UT-TI group as compared with the untreated group. In addition, BT-DMEM BT-TI group showed a significant increased bone mineralization by 57% and 107.4% at 50 and 100 μg/mL, respectively as compared with the untreated group.T hus, the study results concluded that The Trivedi Effect Treatment would be the best alternative treatment for the maintenance of strong and healthy bones and quality of life. Further, it regulates the osteoblast function and improved the level of collagen, ALP, and calcium absorption in wide range of bone disorders along with wide range of adverse bone health conditions.