%0 Journal Article
%T Progesterone and Follicle Stimulating Hormone interact and promote goat preantral follicles survival and development in vitro
%A Lima-Verde
%A Isabel B.
%A Matos
%A Maria H.T.
%A Celestino
%A Juliana J.H.
%A Rossetto
%A Rafael
%A Name
%A Khesller P.O.
%A B¨˘o
%A SŁżnia N.
%A Campello
%A Cl¨˘udio C.
%A Figueiredo
%A Jos¨¦ R.
%J Pesquisa Veterin¨˘ria Brasileira
%D 2012
%I Col¨¦gio Brasileiro de Patologia Animal - CBPA
%R 10.1590/S0100-736X2012000400015
%X we investigated the effects of progesterone and follicle stimulating hormone (fsh) on survival and growth of caprine preantral follicles. pieces of ovarian tissue were cultured for 1 or 7 days in minimum essential medium (mem) alone or containing progesterone (1, 2.5, 5, 10 or 20ng/ml), fsh (50ng/ml) or the interaction between progesterone and fsh. fresh (non-cultured control) and cultured ovarian tissues were processed for histological and ultrastructural studies. after 7 days the addition of fsh to all progesterone concentrations maintained the percentage of normal follicles similar to fresh control. at day 7 of culture, a higher percentage of developing follicles was observed only in 2.5ng/ml of progesterone associated with fsh or 10ng/ml of progesterone alone when compared with control. from day 1 to day 7 of culture, a significant increase in the percentage of developing follicles was observed in mem and 2.5ng/ml of progesterone + fsh. in addition, after 7 days, in all treatments, there was a significant increase in follicular diameter when compared with control, except for mem alone and in 5ng/ml of progesterone + fsh or 10ng/ml of progesterone alone. ultrastructural studies confirmed follicular integrity after 7 days of culture in 2.5ng/ml of progesterone with fsh. in conclusion, this study demonstrated that the interaction between progesterone and fsh maintains ultrastructural integrity, stimulates primordial follicles activation and further growth of cultured caprine preantral follicles.
%K progesterone
%K follicle stimulating hormone
%K fsh
%K preantral follicles
%K goats.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0100-736X2012000400015&lng=en&nrm=iso&tlng=en