%0 Journal Article
%T Concordancia de m¨¦todos para susceptibilidad antimicrobiana en cepas de Mycobacterium tuberculosis aisladas en Monter¨ªa, C¨®rdoba: tubo indicador de crecimiento micobacteriano vs. m¨¦todo de las proporciones m¨²ltiples
%A Mirand
%A Jorge
%A Rios
%A Rodrigo
%A Mattar
%A Salim
%A Alvis
%A Nelson
%J Colombia M¨¦dica
%D 2008
%I Scientific Electronic Library Online
%X objective: to evaluate the agreement of manual mgit (mycobacteria growth indicator tube for antimicrobial susceptibility testing to isoniazid, rifampin, ethambutol and streptomycin) for susceptibility testing vs proportion method on lowenstein-jensen (pm) in mycobacterium tuberculosis strains. methods: a total of forty-five isolates of m. tuberculosis were tested for susceptibility to isoniazid (inh), rifampin (rmp), ethambutol (emb), and streptomycin (sm). the strains were isolated in monter¨ªa, during 2003 and 2005. the agreement between the two assays mentioned was estimated by the kappa test. results: there were thirty-eight strains with identical results while 7 had discrepant results with both methods. the overall resistance to antituberculosis drugs were 33.3% y 31.1% for manual mgit and pm, respectively. mdr was 5 (11.1%) by pm and 4 (8.8%) by manual mgit. the agreement between mgit ast and pm was 95.5% for all drug tested and overall kappa value 0.8374. two discrepancies were found in each drug; with inh and rif (one false resistant and one false susceptible). str and emb (two false susceptibles). turnaround times were 5 to 8 days (median, 6.5 days) for mgit and 20 to 25 days for mp. conclusions: these preliminary data show a good level of agreement between manual mgit ast sire and mp. also mgit is a rapid, easy and efficient method for the drug susceptibility testing of m. tuberculosis.
%K mycobacterium tuberculosis
%K susceptibility
%K isoniazid
%K rifampin
%K ethambutol
%K streptomycin.
%U http://www.scielo.org.co/scielo.php?script=sci_abstract&pid=S1657-95342008000200003&lng=en&nrm=iso&tlng=en