%0 Journal Article
%T Irr1/Scc3 cohesin interacts with Rec8 in meiotic prophase of <i>Saccharomyces cerevisiae</i>
%A Agata Cena
%A Piotr Kowalec
%A Danuta P£żochocka
%A Anna Kurlandzka
%J Open Journal of Genetics
%P 1-6
%@ 2162-4461
%D 2012
%I Scientific Research Publishing
%R 10.4236/ojgen.2012.21001
%X The meiotic cohesin complex of <i>S. cerevisiae</i> shares with the mitotic one the Irr1/Scc3, Smc1, and Smc3 subunits, while the meiosis-specific subunit Rec8 replaces mitotic subunit Scc1/Mcd1. We noticed earlier that the <i>irr</i>1-1 mutation (F658G) severely affected meiosis. The <i>irr</i>1 1/I<i>RR</i>1 cells were entering meiosis before having completed mitotic cell division. Using meiotic two-hybrid assay and co-immunoprecipitation we show that in cells arrested in pachytene due to a lack of a gene-regulatory factor Ndt80, the <i>Irr</i>1 protein interacts with Rec8p and the irr1-1 mutation abolishes this interaction. These findings indicate an important role of Irr1p in early stages of meiosis.
%K Irr1/Scc3
%K Cohesin
%K Rec8
%K Meiosis
%K &lt
%K i&gt
%K Saccharomyces cerevisiae&lt
%K /i&gt
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=17762