%0 Journal Article %T Changes in N2 fixation in Stylosanthes scabra derived from tissue culture %A Valarini %A Maria Jos¨¦ %A Otsuk %A Ivani Pozar %A Vieira %A Maria Lucia Carneiro %J Brazilian Journal of Genetics %D 1997 %I Scientific Electronic Library Online %R 10.1590/S0100-84551997000400025 %X plants were regenerated from leaf-derived callus culture of stylosanthes scabra, a polyploid legume tolerant to drought and adapted to acid soils. a total of 168 regenerants were planted out in leonard jars in a complete randomized design. nitrogen fixation and vegetative growth were indirectly evaluated by shoot dry weight, root dry weight, shoot n content and acetylene reduction activity. the results showed higher variation in the regenerants than in controls not submitted to tissue culture. significant differences were found for all nitrogen fixation related-traits %U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0100-84551997000400025&lng=en&nrm=iso&tlng=en