%0 Journal Article
%T Effectiveness of the BDProbeTec ET system for detection of Mycobacterium tuberculosis complex in sputum and bronchoalveolar lavage specimens
%A Hung
%A Hung-Chang
%A Chan
%A Chi-Ho
%A Tsao
%A Shih-Ming
%A Chen
%A Shiuan-Chih
%A Wu
%A Tzu-Chin
%A Lu
%A Min-Chi
%A Wu
%A Der-Jinn
%A Huang
%A Shu-Ju
%A Lee
%A Yuan-Ti
%J Brazilian Journal of Infectious Diseases
%D 2012
%I Brazilian Society of Infectious Diseases
%R 10.1590/S1413-86702012000300005
%X objective: the diagnostic efficacy of the bdprobetec et mycobacterium tuberculosis (mtb) complex direct detection assay (dtb) performed on bronchoalveolar lavage (bal) specimens and sputum smears was compared with acid-fast bacilli (afb) smear microscopy. method: afb smear microscopy, dtb and culture results of 286 patients with pulmonary tuberculosis were retrospectively reviewed. a total of 120 patients provided expectorated sputum samples, and 166 patients provided bal specimens. culture results and clinical diagnosis were used as gold standards. results: the sensitivity and specificity of the dtb assay in detecting mtb in sputum specimens was significantly higher compared to afb smear microscopy (83.7% and 82.4%, vs. 75.6%, and 41.2%, respectively). the sensitivity and specificity of the dtb assay in detecting mtb in sputum samples was 77.2% and 100% compared to clinical diagnosis, while afb smear had a sensitivity and specificity of 70.3% and 26.3%, respectively. compared to culture, dtb had a sensitivity and specificity of 82.8% and 93.2%, respectively, in detecting mtb from bal specimens; afb smear had a sensitivity and specificity of 41.9% and 87.7%, respectively. compared to clinical diagnosis, dtb had a sensitivity and specificity of 67.2% and 100%, respectively, in detecting mtb from bal specimens; afb smear had a sensitivity and specificity of 34.8% and 79.5%, respectively. conclusions: the superior performance of the dtb assay relative to afb smear microscopy makes it a valuable tool to enable early diagnosis of mtb, thereby improving patient care and reducing transmission.
%K bronchoalveolar lavage
%K mycobacterium tuberculosis
%K acid-fast bacilli staining
%K molecular diagnosis
%K bdprobetec et system.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1413-86702012000300005&lng=en&nrm=iso&tlng=en