%0 Journal Article
%T Partial purification and properties of cellulase-free alkaline xylanase produced by Rhizopus stolonifer in solid-state fermentation
%A Goulart
%A Antonio Jos¨¦
%A Carmona
%A Eleonora Cano
%A Monti
%A Rubens
%J Brazilian Archives of Biology and Technology
%D 2005
%I Tecpar
%R 10.1590/S1516-89132005000300001
%X rhizopus stolonifer was cultivated in wheat bran to produce a cellulase-free alkaline xylanase. the purified enzyme obtained after molecular exclusion chromatography in sephacryl s-200 hr showed optimum temperature as 45o c and hydrolysis phs optima as ph 6.0 and 9.0. xylanase presented higher vmax at ph 9.0 (0.87 ¦Ìmol/mg protein) than at ph 6.0 and minor km at ph 6.0 (7.42 mg/ml) than at ph 9.0.
%K alkaline xylanase
%K rhizopus stolonifer
%K solid-state fermentation.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1516-89132005000300001&lng=en&nrm=iso&tlng=en