%0 Journal Article
%T Cloning and expression of meta-cleavage enzyme (CarB) of carbazole degradation pathway from Pseudomonas stutzeri
%A Larentis
%A Ariane Leites
%A Alves
%A Tito L¨ªvio Moitinho
%A Martins
%A Orlando Bonif¨¢cio
%J Brazilian Archives of Biology and Technology
%D 2005
%I Tecpar
%R 10.1590/S1516-89132005000400016
%X in this work, the 1082bp pcr product corresponding to carbabb genes that encode the heterotetrameric enzyme 2'-aminobiphenyl-2,3-diol 1,2-dioxygenase (carb), involved in the pseudomonas stutzeri atcc 31258 carbazole degradation pathway, was cloned using the site-specific recombination system. recombinant clones were confirmed by pcr, restriction enzyme digestion and sequencing. carb dioxygenase was expressed in high levels and in active form in escherichia coli bl21-si using the his-tagged expression vector pdesttm17 and salt induction for 4h.
%K carbazole
%K 2'-aminobiphenyl-2
%K 3-diol dioxygenase
%K hoada
%K heterologous gene expression
%K petroleum denitrogenation
%K biodegradation.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1516-89132005000400016&lng=en&nrm=iso&tlng=en