%0 Journal Article
%T Scale up of dextran production from a mutant of Pediococcus pentosaceus (SPAm) using optimized medium in a bioreactor
%A Patel
%A Seema
%A Kothari
%A Damini
%A Shukla
%A Rishikesh
%A Das
%A Debasish
%A Goyal
%A Arun
%J Brazilian Archives of Biology and Technology
%D 2011
%I Tecpar
%R 10.1590/S1516-89132011000600007
%X the mutant of pediococcus pentosaceus (spam) produced earlier by uv-mutagenesis exhibiting higher dextransucrase activity as compared to wild-type was used. the generated mutant spam gave 12.2 mg/ml, a 20% higher dextran than wild-type. response surface methodology was carried out for further enhancement of dextran production. to enhance dextran production by the mutant spam, plackett-burman design and a 22 full factorial central composite design was employed. after response optimization, the optimum concentration of sucrose and yeast extract was 5.115% (w/v) and 0.635% (w/v), respectively. the experimental values of dextran 36.0 mg/ml at flask level and 35.0 mg/ml at bioreactor level were in good agreement with the predicted value of 40.8 mg/ml. the increase in dextran production by the mutant spam using the optimized medium was 3 fold higher as compared to unoptimized medium.
%K pediococcus pentosaceus
%K dextran
%K plackett-burman design
%K central composite design
%K response surface methodology.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1516-89132011000600007&lng=en&nrm=iso&tlng=en