%0 Journal Article
%T Validation of a HPLC Method for Determination of Glutamine in Food Additives Using Post-Column Derivatization
%A Marlus Chorilli
%A H¨¦rida Regina Nunes Salgado
%A Fabiana De Santana Santos
%A Luc¨¦lia Magalh£¿es Da Silva
%J American Journal of Analytical Chemistry
%P 113-117
%@ 2156-8278
%D 2012
%I Scientific Research Publishing
%R 10.4236/ajac.2012.32016
%X An ion-exchange chromatography (IEC) followed by post-column derivatization method was validated for the determination of glutamine in food additive products. The chromatographic method was carried out on a sodium cation-exchange column and using detection at 570 nm. The mobile phase consisted of sodium eluant, pH 3.15, with 5% sulfolane, sodium eluant, pH 7.40 and sodium column regenerant. The method was linear in the range of 4 - 50 ¦Ìg/mL (r2 = 0.9999). The accuracy was 99.78%. Moreover, method validation demonstrated acceptable results for precision and robustness. The method was found to be able to use for routine analysis of the glutamine.
%K Glutamine
%K Food Additive Product
%K Liquid Chromatography
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=17283