%0 Journal Article
%T Differences in lipogenesis and lipolysis in obese and non-obese adult human adipocytes
%A CIFUENTES
%A MARIANA
%A ALBALA
%A CECILIA
%A ROJAS
%A CECILIA V
%J Biological Research
%D 2008
%I Sociedad de Biolog¨ªa de Chile
%R 10.4067/S0716-97602008000200009
%X it has been proposed that differences in adipocyte function and/or metabolism between obese and lean individu¨¢is may manifest themselves in functional adipose tissue abnormalities that lead to metabolic disorders in obesity. we studied lipogenesis and lipolysis of omental adipocytes from obese (ob) and non-obese (nob) humans. the specific activity of the lipogenic marker enzyme g3pdh was 50% lower in total adipocytes of ob compared to that of nob subjects. omental adipocytes from ob subjects also had lower basal lipolytic lev¨¦is, and a lower lipolytic response to p-adrenergic stimulus. cholesterol depletion of adipocyte plasma membrane using methyl ¦Â-cyclodextrin caused a lipolytic effect on adipocytes of both groups together, but when obese and lean subjects were analyzed separately, the response was significant only in the obese. we present evidence of a different lipogenic and lipolytic profile in obese individu¨¢is' omental adipocytes, and propose a relevant role of plasma membrane cholesterol, where the impact of its removal in ob and nob adipocyte lipolysis differs.
%K adipocyte
%K cholesterol
%K lipogenesis
%K lipolysis
%K obesity
%K triglyceride metabolism.
%U http://www.scielo.cl/scielo.php?script=sci_abstract&pid=S0716-97602008000200009&lng=en&nrm=iso&tlng=en