%0 Journal Article %T A tridimensional view of the organization of actin filaments in the central nervous system by use of fluorescent photooxidation %A Capani %A Francisco %A Saraceno %A Ezequiel %A Boti %A Valeria Romina %A Aon-Bertolino %A Laura %A Fern¨˘ndez %A Juan Carlos %A Gato %A Fernando %A Krause %A Maria Sol %A Giraldez %A Lisandro %A Ellisman %A Mark H. %A Coirini %A H¨Śctor %J Biocell %D 2008 %I Universidad Nacional de Cuyo %X cellular and subcellular organization and distribution of actin filaments have been studied with various techniques. the use of fluorescence photo-oxidation combined with phalloidin conjugates with eosin has allowed the examination of the precise cellular and subcellular location of f-actin. correlative fluorescence light microscopy and transmission electron microscopy studies of f-actin distribution are facilitated with this method for morphological and physiological studies. because phalloidin-eosin is smaller than other markers, this method allows the analysis of the three-dimensional location of f-actin with high-resolution light microscopy, three-d serial sections reconstructions, and electron tomography. the combination of selective staining and three-dimensional reconstructions provide a valuable tool for revealing aspects of the synaptic morphology that are not available when conventional electron microscopy is used. by applying this selective staining technique and three-dimensional imaging, we uncovered the structural organization of actin in the postsynaptic densities in physiological and pathological conditions. %K f-actin %K phalloidin-eosin phootoxidation %K 3-d reconstructions %K electron tomography. %U http://www.scielo.org.ar/scielo.php?script=sci_abstract&pid=S0327-95452008000100001&lng=en&nrm=iso&tlng=en