%0 Journal Article
%T Desarrollo y validaci車n de un m谷todo por HPLC para la determinaci車n de niveles de vitamina A en leche materna. Su aplicaci車n a una poblaci車n rural de Argentina
%A L車pez
%A Laura B
%A Baroni
%A Andrea V.
%A Rodr赤guez
%A Viviana G
%A Greco
%A Carola B
%A Costa
%A Sara Mac赤as
%A Rodr赤guez de Pece
%A Silvia
%A Ronayne de Ferrer
%A Patricia
%J Archivos Latinoamericanos de Nutrici車n
%D 2005
%I Scientific Electronic Library Online
%X summary. a methodology for the quantification of vitamin a in human milk was developed and validated. vitamin a levels were assessed in 223 samples corresponding to the 5th, 6th and 7th postpartum months, obtained in the province of santiago del estero, argentina. the samples (500 米l) were saponified with potassium hydroxide/ ethanol, extracted with hexane, evaporated to dryness and reconstituted with methanol. a column rp-c18, a mobile phase methanol /water (91:9 v/v) and a fluorescence detector (l excitation 330 nm and l emition 470 nm) were used for the separation and quantification of vitamin a. the analytical parameters of linearity (r2: 0.9995), detection (0.010 米g/ml) and quantification (0.025 米g/ml) limits, precision of the method (relative standard deviation, rsd = 9.0% within a day and rsd = 8.9% among days) and accuracy (recovery = 83.8%) demonstrate that the developed method allows the quantification of vitamin a in an efficient way. the mean values + standard deviation (sd) obtained for the analyzed samples were 0.60 ㊣ 0.32; 0.65 ㊣ 0.33 and 0.61 ㊣ 0.26 米g/ml for the 5th, 6th and 7th postpartum months, respectively. there were no significant differences among the three months studied and the values found were similar to those in the literature. considering the whole population under study, 19.3% showed vitamin a levels less than 0.40 米g/ml, which represents a risk to the children in this group since at least 0.50 米g/ml are necessary to meet the infant daily needs.
%K hplc-validation-retinol
%K human milk..
%U http://www.scielo.org.ve/scielo.php?script=sci_abstract&pid=S0004-06222005000200006&lng=en&nrm=iso&tlng=en