%0 Journal Article %T Identification of species related to Anopheles (Nyssorhynchus) albitarsis by random amplified polymorphic DNA-Polymerase chain reaction (Diptera: Culicidae) %A Wilkerson %A Richard C. %A Gaffigan %A Thomas V. %A Lima %A Jos¨¦ Bento %J Mem¨®rias do Instituto Oswaldo Cruz %D 1995 %I Instituto Oswaldo Cruz, Minist¨¦rio da Sa¨²de %R 10.1590/S0074-02761995000600013 %X species-specific random amplified polymorphic dna-polymerase chain reaction (rapd-pcr) markers were used to identify four species related to anopheles (nyssorhynchus) albitarsis lynch-arrib¨¤lzaga from 12 sites in brazil and 4 in venezuela. in a previous study (wilkerson et al. 1995), which included sites in paraguay and argentina, these four species were designated "a", "b", "c" and "d". it was hypothesized that species a is an. (nys.) albitarsis, species b is undescribed, species c is an. (nys) marajoara galv£¿o and damasceno and species d is an. (nys.) deaneorum rosa-freitas. species d, previously characterized by rapd-pcr from a small sample from northern argentina and southern brazil, is reported here from the type locality of an. (nys.) deaneorum, guajar¨¢-mirim, state of rond£¿nia, brazil. species c and d were found by rapd-pcr to be sympatric at costa marques, state of rond£¿nia, brazil. species a and c have yet to be encountered at the same locality. the rapd markers for species c were found to be conserved over 4,620 km; from iguape, state of s£¿o paulo, brazil to rio socuavo, state of zulia, venezuela. rapd-pcr was determined to be an effective means for the identification of unknown species within this species complex. %K anopheles (nyssorhynchus) albitarsis %K anopheles (nyssorhynchus) marajoara %K anopheles (nyssorhynchus) deaneaorum %K rapd pcr %K identification. %U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0074-02761995000600013&lng=en&nrm=iso&tlng=en