%0 Journal Article
%T The use of ferromagnetic dacron as solid-phase in enzyme immunoassays
%A Le£¿o
%A Ana Maria dos A. Carneiro
%A Carvalho Junior
%A Luiz B.
%A Malague£¿o
%A Elizabeth
%J Mem¨®rias do Instituto Oswaldo Cruz
%D 1994
%I Instituto Oswaldo Cruz, Minist¨¦rio da Sa¨²de
%R 10.1590/S0074-02761994000200012
%X ferromagnetic dacron is proposed as an alternative solid-phase for magnetic enzyme immunoassays. human serum albumin (hsa) was covalentlyimmobilized onto ferromagnetic dacron and as enzyme immunoassay was developed using anti-hsa rabbit sera. peroxidase, o-phenylenediamine (opd) and hydrogen peroxide were used anti-hsa rabbit sera. peroxidase, o-phenylenediamine (opd) and hydrogen peroxide were used as the enzymatic label and substrates, respectively. best results were observed when particles of 63-100 ¦̀m (diameter) and 10 ¦̀g of immobilized antigen were used. positive reactions were detected until dilutions of1:51200 of immune sera. its reproducibility was similar to standard elisa. disruption of the immunocomplexes formed and recuperation of the immobilized antigen in other immunoassays also proved to be reliable.
%K magnetic elisa
%K ferromagnetic dacron
%K human serum albumin.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0074-02761994000200012&lng=en&nrm=iso&tlng=en