%0 Journal Article
%T Bovine papillomavirus type 2 detection in the urinary bladder of cattle with chronic enzootic haematuria
%A Wosiacki
%A Sheila R
%A Claus
%A Marlise P
%A Alfieri
%A Alice F
%A Alfieri
%A Amauri A
%J Mem¨®rias do Instituto Oswaldo Cruz
%D 2006
%I Instituto Oswaldo Cruz, Minist¨¦rio da Sa¨²de
%R 10.1590/S0074-02762006000600009
%X the bovine papillomavirus type 2 (bpv-2) involvement in the aetiology of chronic enzootic haematuria associated to bracken fern ingestion has been suggested for a long time. however, a few reports have shown the presence of the bpv-2 in urinary bladder tumors of cattle. the aim of this study was to investigate the presence of the bpv-2 infection in the urinary bladder of cattle with chronic enzootic haematuria in brazilian cattle herds. sixty-two urinary bladders were collected from adult cattle in beef herds from the north region of the state of paran¨¢, brazil. according to clinical and pathological finds the specimens were distributed in three groups: the group a was constituted by 22 urinary bladders with macroscopic lesions collected at necropsy of cattle with clinical signs of chronic enzootic haematuria; the group b by 30 urinary bladders with macroscopic lesions collected in a slaughterhouse of cows coming from bracken fern-endemic geographical region; and the group c (control) by 10 urinary bladders without macroscopic lesions collected from asymptomatic cattle in a bracken fern-free geographical region. by a semi-nested polymerase chain reaction (pcr) assay, with an internal control, a fragment of the bpv-2 l1 gene with 386 bp length was amplified in 36 (58%) urinary bladder. the rate of bpv-2 positive urinary bladders was 50% (11/22) for group a, 80% (24/30) for group b, and 10% (1/10) for group c (control). the rate of the positive results found in groups a and b that included urinary bladder samples with macroscopic lesions was 67% (35/52) and the detection of the bpv-2 in both groups was significantly higher (p < 0.05) than in the control group. rflp with rsa i and hae iii enzymes evaluated the specificity of the bpv-2 amplicons. the pcr internal control that amplified a 626 bp fragment of the nd5 gene of the bovine mitochondrial genome was amplified in all analyzed samples and excluded false-negatives or invalid results in the semi-nested pcr. these re
%K cattle- viruses
%K urinary bladder
%K chronic enzootic haematuria
%K bovine papillomavirus type 2
%K semi-nested-polymerase chain reaction.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0074-02762006000600009&lng=en&nrm=iso&tlng=en