%0 Journal Article
%T La actividad transcripcional de NF-kB es diferencialmente regulada por dominios especificos del coactivador TIF2
%A Nojek
%A Ignacio M.
%A Werbajh
%A Santiago E.
%A Colo
%A Georgina P.
%A Rubio
%A Fernanda M.
%A Franco
%A Lorena D.
%A Nahmod
%A V¨ªctor E.
%A Costas
%A M¨®nica A.
%J Medicina (Buenos Aires)
%D 2004
%I Fundaci¨®n Revista Medicina
%X we have previously shown that nuclear receptor coactivator overexpression significantly enhanced nf-kb activity in a dose response manner. we studied the mechanism by which tif2 regulates nf-kb activity. we determined that: 1) the p38 specific inhibitor reduces 50% nf-kb transcriptional activity, even in cells that overexpress distinct tif2 deletions; 2) there is a physical interaction between tif2 and p38 and rela determined through in vitro translated protein bindind assays; 3) tif2 is a p38 substrate; 4) there is a physical interaction between tif2 and ikk in tnf-a 20 ng/ml stimulated or not hek 293 cell protein extract, and ikb only in basal conditions, determined by binding pull down assays. this nf-kb complex regulates its activity and targets gene expression in a determined physiologic context depending on the coactivator complex content.
%K nuclear receptor coactivators
%K nf-kb
%K mapk
%K tif2.
%U http://www.scielo.org.ar/scielo.php?script=sci_abstract&pid=S0025-76802004000200008&lng=en&nrm=iso&tlng=en