%0 Journal Article
%T Detecci¨®n fenot¨ªpica y molecular de resistencia a meticilina en S. aureus
%A Castellano-Gonz¨¢lez
%A Maribel J
%A Perozo-Mena
%A Armindo J
%A Vivas-Vega
%A Rosana
%J Kasmera
%D 2008
%I Scientific Electronic Library Online
%X detecting methicillin resistance is complicated due to the heterogeneity of its phenotypic expression, making its detection difficult in the laboratory; this has led to the development of several techniques to increase its expression in vitro. four techniques for detecting methicillin resistance were evaluated: the disk diffusion method with oxacillin (ox, 1 ¦Ìg) and cephoxitin (fox, 30 ¦Ìg); the screening test, minimum inhibitory concentration (mic) and detection of pbp2a, using the presence of meca gen as a reference method; 286 strains of s. aureus, were processed. the sensibility (sen), specificity (spe), positive predictive value (ppv), negative predictive value (npv) and efficiency (efc) of each method were determined. a total of 50 oxacillin resistant, pbp2a positive (meca positive) strains were obtained. sensibility of the ox disk was 99.14%; and of the fox disk was 100%. the sen, ppv, nvp and efc of the other methods were 100%. all the tests, except the ox disk diffusion method (99.14% of esp), were 100% specific.
%K s. aureus
%K methicillin resistance
%K phenotypic methods
%K molecular methods.
%U http://www.scielo.org.ve/scielo.php?script=sci_abstract&pid=S0075-52222008000100004&lng=en&nrm=iso&tlng=en