%0 Journal Article
%T OBTENCI¨®N DE UN SUB-PROTEOMA DE CITOPLASMA DE UNA L¨ªNEA CELULAR DE TROFOBLASTO MEDIANTE FRACCIONAMIENTO CON DETERGENTES
%A Novoa-Herr¨¢n
%A Sandra Susana
%A S¨¢nchez-G¨®mez
%A Myriam
%J Revista de la Academia Colombiana de Ciencias Exactas, F¨ªsicas y Naturales
%D 2011
%I Scientific Electronic Library Online
%X the differential detergent fractionation method described by rambsy and makowski in 1999, was modified using a digitonin (0.015%) buffer to permeate htr8/svneo human trophoblast cells and obtain the cytoplasm proteins in the soluble fraction, while membrane proteins were dissolved from the pellet with triton x-100 (0.5%) buffer. we obtained a reproducible membrane-free cytoplasm protein extract suitable for 2-de, by chloroform/methanol precipitation. in conclusion, the method here described is appropriate for the separation of cytoplasm sub-proteomes from cell cultures, with an optimal reproducibility and recovery to perform proteomic profile analysis by bidimensional electrophoresis.
%K proteomic
%K cell fractionation
%K triton x-100
%K digitonine
%K trophoblast
%K htr8/svneo.
%U http://www.scielo.org.co/scielo.php?script=sci_abstract&pid=S0370-39082011000300001&lng=en&nrm=iso&tlng=en