%0 Journal Article
%T Amplificaci¨®n interespec¨ªfica de microsat¨¦lites de Astyanax abramis, utilizando cebadores para la especie emparentada Astyanax fasciatus
%A Ojeda
%A Alejandra Paola
%A Hirt
%A Lourdes M.
%A Zapata
%A Pedro Dar¨ªo
%J Revista de ciencia y tecnolog£¿-a
%D 2012
%I Scientific Electronic Library Online
%X the yabot¨ª biosphere reserve is located in the center-east of misiones (argentina) and it covers a vast area of the basins of yabot¨ª streams, tributaries of alto uruguay river. astyanaxis important in the trophic level and in the maintenance of the natural fish communities, since it is food for the last links of the food chain. population analysis are important ecological data for supporting the reasonable use of the resource. futhermore, there are no yet specific data for ssr regions in astyanax abramis, only for astyanax fasciatus. the objective of this work was to cross-amplify the microsatellites regions of in astyanaxabramiswith specific primers designed for a. fasciatus. dna was extracted from the caudal fin peduncle from 13 specimens. the pcr conditions were standardized to extend 3 microsatellite regions in a. abramis: abral, abra2, abra3 y abra4. three of these were sequenced and registered in the genebank database. although our findings are preliminary, they confirm the transferability of the use of primers, identifying sequences preserved and allowing us to establish the first microsatellite sequence of the species under study.
%K astyanax abramis
%K ssr
%K cross-amplification.
%U http://www.scielo.org.ar/scielo.php?script=sci_abstract&pid=S1851-75872012000100007&lng=en&nrm=iso&tlng=en