%0 Journal Article
%T Studies on whole cell fluorescence-based screening for epoxide hydrolases and Baeyer-Villiger monoxygenases
%A Bicalho
%A Beatriz
%A Chen
%A Lu S.
%A Grognux
%A Johann
%A Reymond
%A Jean-Louis
%A Marsaioli
%A Anita J.
%J Journal of the Brazilian Chemical Society
%D 2004
%I Sociedade Brasileira de Qu¨ªmica, SBQ
%R 10.1590/S0103-50532004000600019
%X biocatalysis reactions were performed on microtiter plates (200 ¦̀l) aiming at the utilization of fluorogenic substrates (100 ¦̀mol l-1) for rapid whole cell screening for epoxide hydrolases (ehs) and baeyer-villiger monoxygenases (bvmos). a final protocol was achieved for ehs, with 3 new enzymatic sources being detected (agrobacterium tumefaciens, pichia stipitis, trichosporom cutaneum). the fluorogenic assay for bvmo did not work as expected. however, an approach to possible variables involved (aeration; ph) provided the first detection of a bvmo activity in t. cutaneum.
%K microorganisms
%K baeyer-villiger monoxygenase
%K epoxide hydrolase.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0103-50532004000600019&lng=en&nrm=iso&tlng=en