%0 Journal Article
%T On-line identification of minor flavones from sugarcane juice by LC/UV/MS and post-column derivatization
%A Colombo
%A Renata
%A Yariwake
%A Janete H.
%A Queiroz
%A Emerson F.
%A Ndjoko
%A Karine
%A Hostettmann
%A Kurt
%J Journal of the Brazilian Chemical Society
%D 2009
%I Sociedade Brasileira de Qu¨Şmica, SBQ
%R 10.1590/S0103-50532009000900003
%X this work describes the on-line characterization of minor flavones from sugarcane (saccharum officinarum) juice by high-performance liquid chromatography coupled to diode array uv detection and mass spectrometry (lc/uv/ms) using atmospheric pressure chemical ionization-collision-induced dissociation (apci-cid-ms/ms) and post-column derivatization using uv shift reagents. hplc-uv analysis with shift reagents provided information about the substitution pattern in the flavonoid skeleton and, combined with ms data, these techniques allowed for the on-line identification of five "garapa" flavones: luteolin-8-c-glucosyl-7-o-glucuronide; tricin-7-o-neohesperoside-4'-o-rhamnoside; tricin-7-o-methylglucuronate-4'-o-rhamnoside; tricin-7-o-methylglucuronide; swertisin, while four other compounds were partially identified as glycosylflavones. only swertisin (7-o-methylapigenin-6-c-glucoside) was reported previously in sugarcane molasses.
%K sugarcane juice
%K saccharum officinarum l. (poaceae)
%K flavones
%K lc/uv/ms
%K uv shift reagents.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0103-50532009000900003&lng=en&nrm=iso&tlng=en