%0 Journal Article
%T Gel Permeation Chromatography Purification and Gas Chromatography-Mass Spectrometry Detection of Multi-Pesticide Residues in Traditional Chinese Medicine
%A Wan-E Zhuang
%A Zhen-Bin Gong
%J American Journal of Analytical Chemistry
%P 24-32
%@ 2156-8278
%D 2012
%I Scientific Research Publishing
%R 10.4236/ajac.2012.31005
%X The measurement of 23 organochlorine, organophosphorus, and pyrethroid pesticides in typical traditional Chinese medicine (TCM), flos lonicerae, was made using gel permeation chromatography (GPC) purification and gas chroma- tography-mass spectrometry (GC-MS) detection. The pesticides were extracted with ultrasonic device and 5.0 mL mixture of ethyl acetate and cyclohexane (1:1, v/v). Coextractants from sample matrices which may have interfere to the qualitative and quantitative analysis, such as pigments, were removed using GPC purification. Simultaneous full scan and selective ion monitor (scan/SIM) mode for GC-MS was used for qualitative and quantitative analysis, which pro- vided retention time and characteristic fragments ratio for each pesticide so as to positively identify each analyte. Rela- tive standard deviations (RSDs) were within 7.7% (5.0 - 22.5 ¦Ìg/kg, n = 3). The recoveries of pesticide standards at the spiked concentration of 5.0 - 22.5 ¦Ìg/kg were between 87.1% and 110.9%. Limits of detection (LODs) for the analytes were 0.16 - 3.2 ¦Ìg/kg, which could meet the demand of routine analysis and TCM quality control.
%K Traditional Chinese Medicine
%K Multi-Pesticide Residue
%K Flos Lonicerae
%K Gel Permeation Chromatography
%K Gas Chromatography-Mass Spectrometry
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=16811