%0 Journal Article
%T A novel Kv1.1 potassium channel blocking toxin from the venom of Palamneus gravimanus (Indian black scorpion)
%A More
%A S. S.
%A Mirajkar
%A K. K.
%A Gadag
%A J. R.
%A Menon
%A K. S.
%A Mathew
%A M. K.
%J Journal of Venomous Animals and Toxins including Tropical Diseases
%D 2005
%I Centro de Estudos de Venenos e Animais Pe?onhentos - CEVAP, Universidade Estadual Paulista - UNESP
%R 10.1590/S1678-91992005000300009
%X a peptide toxin was isolated from the venom of palamneus gravimanus, the indian black scorpion, to block human kv1.1 channels expressed in xenopus laevis oocytes. a 4.5 kd peptide (toxin), as confirmed by sds-page, was purified to homogeneity by ion exchange chromatography using cm-sephadex c-25 followed by sephadex g-50 gel filtration. palamneus gravimanus toxin (pgt) selectively blocks the human cloned voltage-gated potassium channel hkv1.1 in a two-electrode voltage-clamp (tevc) technique. the results obtained indicate that the toxin blocks the hkv1.1 channel at a nanomolar concentration range (ki value of 10 nm) of the peptide to the external side of the cell. the blockage seems to be voltage-dependent. comparative structure of pgt (a 4.5 kd peptide) with btk-2 suggests a close relationship; therefore this toxin can be employed to investigate the hkv1.1 channel structure.
%K hkv1.1
%K k+ channel
%K pgt
%K tevc
%K palamneus gravimanus
%K xenopus laevis oocytes
%K indian black scorpion.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1678-91992005000300009&lng=en&nrm=iso&tlng=en