%0 Journal Article
%T Biotin-avidin sandwich elisa with specific human isotypes IgG1 and IgG4 for Culicidae mosquito blood meal identification from an epizootic yellow fever area in Brazil
%A Marass¨¢
%A AM
%A Paula
%A MB
%A Gomes
%A AC
%A Consales
%A CA
%J Journal of Venomous Animals and Toxins including Tropical Diseases
%D 2009
%I Centro de Estudos de Venenos e Animais Pe?onhentos - CEVAP, Universidade Estadual Paulista - UNESP
%R 10.1590/S1678-91992009000400008
%X with a view toward investigating the feeding behavior of culicidae mosquitoes from an area of epizootic yellow fever transmission in the municipalities of garruchos and santo ant£¿nio das miss£¿es, rio grande do sul state, brazil, specimens were collected by aspiration from september 2005 to april 2007. the engorged females were submitted to blood meal identification by enzyme-linked immunosorbent assay (elisa). a total of 142 blood-engorged samples were examined for human or monkey blood through species-specific igg. additional tests for specificity utilizing isotypes igg1 and igg4 of human monoclonal antibodies showed that only anti-human igg1 was effective in recognizing blood meals of human origin. the results indicated a significant difference (p = 0.027) in detection patterns in samples of haemagogus leucocelaenus recorded from human blood meals at santo ant£¿nio das miss£¿es, which suggests some degree of exposure, since it was an area where epizootic outbreaks have been reported.
%K culicidae
%K elisa
%K feeding habits
%K isotypes
%K yellow fever.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1678-91992009000400008&lng=en&nrm=iso&tlng=en