%0 Journal Article
%T Characterization of Clostridium perfringens isolated from mammals and birds from Guwahati city, India
%A Rahman
%A Mafruza S
%A Sharma
%A RK
%A Borah
%A P
%A Chakraborty
%A A
%A Devi
%A Mandakini RK
%A Longjam
%A N
%J Journal of Venomous Animals and Toxins including Tropical Diseases
%D 2012
%I Centro de Estudos de Venenos e Animais Pe?onhentos - CEVAP, Universidade Estadual Paulista - UNESP
%R 10.1590/S1678-91992012000100010
%X of the 102 samples collected from mammals and birds, both domestic and captive wild, 48 were found to be positive for clostridium perfringens. most of the mammal isolates (84.38%) appeared to have been collected from clinically affected animals, while 33.33% of the bird samples were from clinically affected and 21.43% from apparently healthy birds infected with c. perfringens. isolates revealed high sensitivity to ciprofloxacin, enrofloxacin and norfloxacin. among the isolated c. perfringens, 30 (62.50%) showed dnase production. hemolytic activity was recorded in 14 (24.16%) of the isolates and 28 (58.33%) showed phospholipase c production. all the phospholipase c positive isolates revealed the presence of cpa gene encoding alpha (¦Á) toxin. of the 102 samples collected from mammals and birds, both domestic and captive wild, 48 were found to be positive for clostridium perfringens. most of the mammal isolates (84.38%) appeared to have been collected from clinically affected animals, while 33.33% of the bird samples were from clinically affected and 21.43% from apparently healthy birds infected with c. perfringens. isolates revealed high sensitivity to ciprofloxacin, enrofloxacin and norfloxacin. among the isolated c. perfringens, 30 (62.50%) showed dnase production. hemolytic activity was recorded in 14 (24.16%) of the isolates and 28 (58.33%) showed phospholipase c production. all the phospholipase c positive isolates revealed the presence of cpa gene encoding ¦Á toxin.
%K clostridium perfringens
%K pcr
%K ¦Á toxin
%K cpa gene
%K polymerase chain reaction.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1678-91992012000100010&lng=en&nrm=iso&tlng=en