%0 Journal Article
%T Kinetics of venom and antivenom serum levels, clinical evaluation and therapeutic effectiveness in dogs inoculated with Crotalus durissus terrificus venom
%A Sangiorgio
%A F.
%A Sakate
%A M.
%A Nogueira
%A R. M. B.
%A ArašČjo JR.
%A J. P.
%A Chavez-Olortegui
%A C.
%J Journal of Venomous Animals and Toxins including Tropical Diseases
%D 2008
%I Centro de Estudos de Venenos e Animais Pe?onhentos - CEVAP, Universidade Estadual Paulista - UNESP
%R 10.1590/S1678-91992008000100008
%X this work evaluated the clinical and therapeutic aspects as well as serum levels of venom and antivenom igg by enzyme-linked immunosorbent assay (elisa) in experimental envenomation of dogs with crotalus durissus terrificus venom. twenty-eight mixed breed adult dogs were divided into four groups of seven animals each, group i: only venom; group ii, venom + 50 ml of anti-bothropic-crotalic serum (50mg) + fluid therapy; group iii, venom + 50 ml of anti-bothropic-crotalic serum + fluid therapy + urine alkalination; group iv, 50 ml of anti-bothropic-crotalic serum. the lyophilized venom of crotalus durissus terrificus was reconstituted in saline solution and subcutaneously inoculated at the dose of 1mg/kg body weight. the dogs presented clinical signs of local pain, weakness, mandibular ptosis, mydriasis, emesis and salivation. the venom levels detected by elisa ranged from 0 to 90ng/ml, according to the severity of the clinical signs. serum antivenom ranged from 0 to 3ug/ml and was detected for up to 138h after treatment. elisa results showed the effectiveness of the serum therapy for the venom neutralization.
%K crotalus durissus terrificus
%K dogs
%K clinical signs
%K elisa.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1678-91992008000100008&lng=en&nrm=iso&tlng=en