%0 Journal Article
%T Diversity and three-dimensional structures of the alpha Mcr of the methanogenic Archaea from the anoxic region of Tucuru¨ª Lake, in Eastern Brazilian Amazonia
%A Santana
%A Priscila Bessa
%A Ghilardi Junior
%A Rubens
%A Alves
%A Claudio Nahum
%A Silva
%A Jeronimo Lameira
%A McCulloch
%A John Anthony
%A Schneider
%A Maria Paula Cruz
%A Silva
%A Artur da Costa da
%J Genetics and Molecular Biology
%D 2012
%I Sociedade Brasileira de Gen¨¦tica
%R 10.1590/S1415-47572012005000004
%X methanogenic archaeans are organisms of considerable ecological and biotechnological interest that produce methane through a restricted metabolic pathway, which culminates in the reaction catalyzed by the methyl-coenzyme m reductase (mcr) enzyme, and results in the release of methane. using a metagenomic approach, the gene of the a subunit of mcr (mcr¦Á) was isolated from sediment sample from an anoxic zone, rich in decomposing organic material, obtained from the tucuru¨ª hydroelectric dam reservoir in eastern brazilian amazonia. the partial nucleotide sequences obtained were 83 to 95% similar to those available in databases, indicating a low diversity of archaeans in the reservoir. two orders were identified -the methanomicrobiales, and a unique operational taxonomic unit (otu) forming a clade with the methanosarcinales according to low bootstrap values. homology modeling was used to determine the three-dimensional (3d) structures, for this the partial nucleotide sequence of the mcr¦Á were isolated and translated on their partial amino acid sequences. the 3d structures of the archaean mcr¦Á observed in the present study varied little, and presented approximately 70% identity in comparison with the mcr¦Á of methanopyrus klanderi. the results demonstrated that the community of methanogenic archaeans of the anoxic c1 region of the tucurui reservoir is relatively homogeneous.
%K amazonian
%K archaea
%K diversity
%K methyl-coenzyme m reductase
%K mcr¦Á
%K homology modeling.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1415-47572012000100018&lng=en&nrm=iso&tlng=en