%0 Journal Article %T ¦Á-Tocopherol levels in natural and artificial aging of soybean seeds %A Giurizatto %A Maria Izabel Kr¨¹ger %A Ferrarese-Filho %A Osvaldo %A Ferrarese %A Maria de Lourdes Lucio %A Robaina %A Antonio Dias %A Gon£¿alves %A Manoel Carlos %A Cardoso %A Cl¨¢udia Andr¨¦a Lima %J Acta Scientiarum. Agronomy %D 2012 %I Scientific Electronic Library Online %R 10.4025/actasciagron.v34i3.12660 %X tocopherols are well known constituents of vitamin e, and the main antioxidants in soybean. are natural antioxidants and stabilizers that can inhibit lipid degradation, reducing non-enzymatic oxidation of these compounds during storage of seeds, germination and initial development of seedlings. the objective of this work was to determine the level of ¦Á-tocopherol in four soybean seeds cultivars naturally and artificially aged. seeds of four soybean cultivars stored from 0 to 180 days in a dry chamber (natural aging) and subjected to high temperature and humidity (artificial aging) were analyzed for ¦Á-tocopherol content. the quantification of ¦Á-tocopherol in the soybean seeds was performed by high performance liquid chromatography (hplc) combined with the soxhlet extraction method. significant differences in ¦Á-tocopherol levels in seeds were observed for all cultivars and storage times. the ¦Á-tocopherol contents of the soybean seeds showed linear correlations with an increasing period of storage for all the cultivars studied. however, the artificially aged seeds had a higher content of ¦Á-tocopherol than those naturally aged. %K glycine max (l.) merrill %K ¦Á-tocopherol %K storage. %U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1807-86212012000300015&lng=en&nrm=iso&tlng=en