%0 Journal Article
%T N-acetylcysteine and fructose-1,6-bisphosphate: immunomodulatory effects on mononuclear cell culture
%A Mello
%A Ricardo Obalski de
%A Lunardelli
%A Adroaldo
%A Caberlon
%A Eduardo
%A Moraes
%A Cristina Machado Bragan£¿a de
%A Santos
%A Roberto Christ Vianna
%A Costa
%A Vin¨ªcius Lorini da
%A da Silva
%A Gabriela Viegas
%A Scherer
%A Patr¨ªcia da Silva
%A Buaes
%A Luiz Eduardo Coimbra
%A Donadio
%A M¨¢rcio Vin¨ªcius Fagundes
%A Nunes
%A Fernanda Bordignon
%A Oliveira
%A Jarbas Rodrigues de
%J Jornal Brasileiro de Patologia e Medicina Laboratorial
%D 2012
%I Sociedade Brasileira de Patologia Cl¨ªnica
%R 10.1590/S1676-24442012000200007
%X introduction: sepsis is a complex syndrome caused by an uncontrolled systemic inflammatory response. inflammatory cytokines play a pivotal role in septic shock pathogenesis. therapeutic strategies have been tested in order to modulate the excessive generation or function of sepsis mediators. objective: the objective of the present study was to investigate the therapeutic effect of n-acetylcysteine (nac) and its association with fructose-1,6-bisphosphate (fbp) on t-lymphocytes proliferation, interleukin-1¦Â (il-1¦Â) and monocyte chemotactic protein-1 (mcp-1) levels. material and methods: peripheral blood mononuclear cell samples were isolated from healthy individuals. t-lymphocytes were stimulated with phytohemagglutinin for 96 hours and submitted to different concentrations of nac or nac associated with fbp. results: nac (10 and 15 mm) and nac (15 mm) associated with fbp reduced t-lymphocytes proliferation. il-1¦Â levels rose in the presence of both nac (15 mm) and nac with fbp (1.25 mm). mcp-1 levels were reduced only by nac (15 mm) associated with fbp (1.25 mm). conclusion: the results suggest that both nac itself and nac associated with fbp inhibit cellular proliferation, acting as potent immunomodulatory agents, which corroborates its use in the treatment of inflammatory diseases.
%K fructose-1
%K 6-bisphosphate
%K n-acetylcysteine
%K t-lymphocytes
%K interleukin-1¦Â
%K mcp-1.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1676-24442012000200007&lng=en&nrm=iso&tlng=en