%0 Journal Article
%T CD63 e CD123 express£¿o, autoanticorpos IgG e acur¨¢cia do teste do soro aut¨®logo em pacientes com urtic¨¢ria cr£¿nica
%A Calamita
%A Zamir
%A Antunes
%A Roseli Nunes da Silveira
%A Almeida Filho
%A Odilon Marques de
%A Baleotti J¨²nior
%A Wilson
%A Calamita
%A Andrea Bronhara Pel¨¢
%A Fukasawa
%A Josianne Thomazini
%A Cavaretto
%A D¨¦bora de Aguiar
%J Jornal Brasileiro de Patologia e Medicina Laboratorial
%D 2012
%I Sociedade Brasileira de Patologia Cl¨ªnica
%R 10.1590/S1676-24442012000100005
%X introduction: the autologous serum skin test (asst) may suggest an autoimmune etiology in chronic urticaria (cu). a new laboratory technique called basophil activation test (bat) has been currently employed for its diagnosis. objective: to analyze asst in relation to bat as well as to evaluate interleukin 3 (il3) receptors (cd123) and non-specific immunoglobulin g (igg) autoantibodies bound to basophils in patients with chronic urticaria. methods: we studied 33 adults with cu and mean age of 42.5 + 14 years. after stimulation by serum from patients with cu, cd63 expression on basophils from one atopic donor was analyzed by flow cytometry. furthermore, we investigated cd123 and igg autoantibody expressions. results: the odds ratio (or) between asst and bat was 1.00 (95% confidence interval [ci]: 0.22 to 4.5). the asst for autoimmune cu diagnosis showed an accuracy of 54.5%, sensitivity of 66%, specificity of 33%, positive predictive value of 63%, and negative predictive value of 36%. there was no statistical difference between the studied groups as to mean non-specific igg and cd123 expressions (for a p < 0.05). discussion: this study demonstrated that asst has low accuracy in the diagnosis of autoimmune cu. concerning other analyzed aspects, there was no statistical difference between positive asst and negative asst. conclusions: due to insufficient studies in this area and the relevance of this issue, further investigation is required.
%K chronic urticaria
%K autologous serum skin test
%K basophil activation test
%K accuracy
%K cd63
%K cd123.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1676-24442012000100005&lng=en&nrm=iso&tlng=en