%0 Journal Article
%T Calcium citrate improves the epithelial-to-mesenchymal transition induced by acidosis in proximal tubular cells
%A Cabalgante
%A Maria Jos¨¦ Rodriguez
%A Gadola
%A Liliana
%A Luzardo
%A Leonella
%A M¨¢rquez
%A Mar¨ªa
%A Boggia
%A Jos¨¦
%A Boim
%A Mirian Aparecida
%J Jornal Brasileiro de Nefrologia
%D 2012
%I Sociedade Brasileira de Nefrologia
%R 10.5935/0101-2800.20120023
%X introduction: epithelial-to-mesenchymal transition (emt) is a key event in renal fibrosis. the aims of the study were to evaluate acidosis induced emt, transforming-growth-factor (tgf) ¦Â1 role and citrate effect on it. methods: hk2 cells (atcc 2290) were cultured in dmem/ham f12 medium, ph 7.4. at 80% confluence, after 24 hr under serum free conditions, cells were distributed in three groups (24 hours): a) control: ph 7.4, b) acidosis: ph 7.0 and c) calcium citrate (0.2 mmol/l) + ph 7.0. change (¦Ä) of intracellular calcium concentration, basal and after angiotensin ii (10-6m) exposition, were measured to evaluate cellular performance. emt was evaluated by the expression of ¦Á-smooth muscle actin (¦Á-sma) and e-cadherin by immunocytochemistry and/or western blot. tgf-¦Â1 secretion was determined by elisa in cell supernatant. results: at ph 7.0 hk2 cells significantly reduced e-cadherin and increased ¦Á-sma expression (emt). supernatant tgf-¦Â1 levels were higher than in control group. calcium citrate decreased acidosis induced emt and improved cells performance, without reduction of tgf-¦Â production. conclusions: acidosis induces emt and secretion of tgf-¦Â1 in tubular proximal cells in culture and citrate improves cellular performance and ameliorates acidosis induced emt.
%K acidosis
%K citrates
%K epithelium.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0101-28002012000400006&lng=en&nrm=iso&tlng=en